对来自阿根廷的苏云金芽孢杆菌分离株中的cry2基因进行筛选。

Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina.

作者信息

Sauka Diego H, Cozzi Jorge G, Benintende Graciela B

机构信息

Area Bioinsumos Microbianos, Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, De los Reseros y Las Cabañas, Castelar, Buenos Aires, Argentina.

出版信息

Antonie Van Leeuwenhoek. 2005 Aug;88(2):163-5. doi: 10.1007/s10482-005-3368-2.

DOI:10.1007/s10482-005-3368-2

PMID:16096692

Abstract

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identification of cry2 genes from Bacillus thuringiensis (Bt) was established. Strains from different sources of Argentina were analyzed to study the distribution of cry2 genes. The results showed that cry2Aa/cry2Ab profile was the most abundant irrespective of source and represented 56 of 59 Bt isolates (94.9%). Three different cry2 profiles were found in this collection, one of which was novel.

摘要

建立了一种用于鉴定苏云金芽孢杆菌（Bt）cry2基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法。对来自阿根廷不同来源的菌株进行分析，以研究cry2基因的分布。结果表明，无论来源如何，cry2Aa/cry2Ab图谱最为丰富，在59株Bt分离株中有56株（94.9%）呈现该图谱。在这个菌株集合中发现了三种不同的cry2图谱，其中一种是新的。