Vilas-Bôas Gislayne Trindade, Lemos Manoel Victor Franco
Departamento de Biologia Aplicada à Agropecuária, FCAV/UNESP, Via de Acesso Prof. Paulo D. Castellane, Jaboticabal/SP, 14884-900, Brazil.
Can J Microbiol. 2004 Aug;50(8):605-13. doi: 10.1139/w04-052.
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were selected according to their geographic origin for genetic characterization with the 16S rRNA gene, RAPD, and plasmid profile. Isolates containing cry1 genes were the most abundant (48%) followed by the cry11 and cyt (7%) and cry8 genes (2%). Finally, 40.3% of the isolates did not produce any PCR product. The plasmid profile and RAPD analysis showed a remarkable diversity among the isolates of B. thuringiensis not observed in the 16S rRNA gene. These results suggest that the genetic diversity of B. thuringiensis species results from the influence of different ecological factors and spatial separation between strains generated by the conquest of different habitats.
通过使用针对不同cry和cyt基因的特异性引物进行PCR，对来自巴西的218株苏云金芽孢杆菌分离株进行了晶体蛋白基因鉴定。在这些分离株中，根据其地理来源选择了95株，用16S rRNA基因、随机扩增多态性DNA（RAPD）和质粒图谱进行遗传特征分析。含有cry1基因的分离株最为丰富（48%），其次是cry11和cyt基因（7%）以及cry8基因（2%）。最后，40.3%的分离株未产生任何PCR产物。质粒图谱和RAPD分析表明，苏云金芽孢杆菌分离株之间存在显著的多样性，而在16S rRNA基因中未观察到这种情况。这些结果表明，苏云金芽孢杆菌物种的遗传多样性是由不同生态因素的影响以及菌株因占据不同栖息地而产生的空间分离所致。
