Agrawal R, Joseph R
Food Microbiology Department, Central Food Technological Research Institute, Mysore, India.
Appl Microbiol Biotechnol. 2000 Mar;53(3):335-7. doi: 10.1007/s002530050030.
Resting cells of a locally isolated strain of Aspergillus niger caused the bioconversion of alpha pinene to verbenone. The formation of verbenone was raised from trace amounts (under screening conditions) to 3.28 mg/100 ml (equivalent to a molar yield of 16.5% conversion of the substrate) by amending the cultivation medium for the fungus. The optimal conditions were: 6 g/100 ml for the glucose concentration, a pH of 7.0, an alpha pinene concentration of 20 mg/100 ml, and a 6-h incubation period for the reaction.
一株本地分离的黑曲霉菌株的静止细胞可将α-蒎烯生物转化为马鞭草烯酮。通过调整真菌的培养基，马鞭草烯酮的生成量从痕量（在筛选条件下）提高到了3.28毫克/100毫升（相当于底物转化率的摩尔产率为16.5%）。最佳条件为：葡萄糖浓度6克/100毫升、pH值7.0、α-蒎烯浓度20毫克/100毫升以及反应孵育期6小时。
