黑曲霉将芳樟醇生物转化为呋喃型和吡喃型芳樟醇氧化物。

Biotransformation of linalool to furanoid and pyranoid linalool oxides by Aspergillus niger.

作者信息

Demyttenaere J C, Willemen H M

机构信息

Department of Organic Chemistry, Faculty of Agricultural and Applied Biological Sciences, Gent, Belgium.

出版信息

Phytochemistry. 1998 Mar;47(6):1029-36. doi: 10.1016/s0031-9422(97)00688-2.

DOI:10.1016/s0031-9422(97)00688-2

PMID:9564732

Abstract

Biotransformation of (+/-)-linalool with submerged shaking cultures of Aspergillus niger, particularly A. niger ATCC 9142, yielded a mixture of cis- and trans-furanoid linalool oxide (yield 15-24%) and cis- and trans-pyranoid linalool oxide (yield 5-9%). Biotransformation of (R)-(-)-linalool with the same strain yielded almost pure trans-furanoid and trans-pyranoid linalool oxide (ee > 95). These conversions were purely biocatalytic, since in acidified water (pH < 3.5) almost 50% linalool was recovered unchanged, the rest was lost by evaporation. The biotransformation was also carried out with growing surface cultures.

摘要

用黑曲霉，特别是黑曲霉ATCC 9142的深层振荡培养物对（±）-芳樟醇进行生物转化，得到顺式和反式呋喃型芳樟醇氧化物的混合物（产率15 - 24%）和顺式和反式吡喃型芳樟醇氧化物的混合物（产率5 - 9%）。用同一菌株对（R）-（-）-芳樟醇进行生物转化，得到几乎纯的反式呋喃型和反式吡喃型芳樟醇氧化物（对映体过量>95）。这些转化完全是生物催化的，因为在酸化水中（pH < 3.5），几乎50%的芳樟醇未发生变化而被回收，其余部分因蒸发而损失。生物转化也在生长的表面培养物中进行。