Wanachiwanawin D, Manatsathit S, Lertlaituan P, Thakerngpol K, Suwanagool P
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1998 Dec;29(4):767-71.
Microsporidia have been recognized as emerging opportunistic agents affecting multiple organs. Intestinal microsporidiosis caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis is a common disease which is associated with gastrointestinal symptoms, particularly in AIDS patients. So far, information on the frequency of this enteric disease in Thailand is not available. Therefore, the present study was undertaken to investigate the prevalence of intestinal microsporidiosis in HIV infected persons with chronic diarrhea. From 1995 to 1996, multiple diarrheal stool specimens were received and examined for the presence of the organism using Weber's modified trichrome staining method and transmission electron microscopy for confirmation. Twenty-two of 66 patients (33.3%) were positive for microsporidia which appeared as pink-red spores of 0.8-1.2 x 0.7-0.9 microm with the characteristic transverse or oblique band representing the coiled polar filament. Clinical features of these patients included chronic diarrhea (100%), weight loss (100%), abdominal pain (77%), fever (36%), vomiting (36%) and anorexia (18%). Transmission electron microscopic examination of fecal specimens from the 22 patients with positive staining results revealed E. bieneusi in 18 cases.
微孢子虫已被确认为影响多个器官的新兴机会性病原体。由比氏肠微孢子虫和肠脑炎微孢子虫引起的肠道微孢子虫病是一种常见疾病,与胃肠道症状有关,尤其是在艾滋病患者中。到目前为止,泰国这种肠道疾病的发病频率尚无相关信息。因此,本研究旨在调查慢性腹泻的艾滋病毒感染者中肠道微孢子虫病的患病率。1995年至1996年,收集了多个腹泻粪便标本,并使用韦伯改良三色染色法检查是否存在该病原体,并用透射电子显微镜进行确认。66例患者中有22例(33.3%)微孢子虫呈阳性,微孢子虫表现为大小为0.8 - 1.2×0.7 - 0.9微米的粉红色至红色孢子,具有代表盘绕极丝的特征性横向或斜向带。这些患者的临床特征包括慢性腹泻(100%)、体重减轻(100%)、腹痛(77%)、发热(36%)、呕吐(36%)和厌食(18%)。对22例染色结果呈阳性的患者粪便标本进行透射电子显微镜检查,发现18例患者感染了比氏肠微孢子虫。