缅甸人体隐孢子虫的首次鉴定和基因分型。
First identification and genotyping of Enterocytozoon bieneusi in humans in Myanmar.
机构信息
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, MOH, Shanghai, 200025, China.
Department of Parasitology, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
出版信息
BMC Microbiol. 2020 Jan 13;20(1):10. doi: 10.1186/s12866-019-1694-1.
BACKGROUND
Intestinal pathogen infections are widespread among impoverished populations. Enterocytozoon bieneusi is the most common pathogen of intestinal microsporidian species in humans worldwide. However, no epidemiological information is available on E. bieneusi infection in humans in Myanmar. The present study comprised the first identification and genotyping of E. bieneusi in humans conducted in Myanmar.
RESULTS
A total of 172 fecal specimens were collected from the Wa people (one each) in four villages of Pangsang Township of the Matman District of Shan State, Myanmar, and each participant completed a questionnaire. E. bieneusi was identified and genotyped using polymerase chain reaction (PCR) and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. The average prevalence of E. bieneusi was 8.72% (15/172), ranging from 3.85 to 13.89% by village. E. bieneusi infection was not related to any of the risk factors studied. Six genotypes were identified, comprising two known genotypes Peru6 (n = 10) and D (n = 1) and four novel genotypes (MMR23, MMR25, MMR86, and MMR87) (one each), and two people infected with genotype Peru6 were from the same family. A phylogenetic analysis based on a neighbor-joining tree of the ITS sequences of E. bieneusi indicated that all the six genotypes were clustered into group 1.
CONCLUSIONS
This is the first identification and genotyping of E. bieneusi in humans in Myanmar. The observations that the two people infected with genotype Peru6 were from the same family, and that all six genotypes obtained in the present study fell into zoonotic group 1, showed the potential for anthropogenic and zoonotic transmissions. The present data argue for the importance of epidemiological control and prevention from medical sectors.
背景
肠道病原体感染在贫困人群中广泛存在。肠微孢子虫中的贝氏肠微孢子虫是全世界人类中最常见的肠道病原体。然而，目前尚无缅甸人类肠微孢子虫感染的流行病学信息。本研究首次对缅甸人类肠微孢子虫进行了鉴定和基因分型。
结果
从缅甸掸邦马当区潘桑镇四个村庄的 172 名瓦族人（每人 1 份）中采集了粪便标本，每位参与者都填写了一份问卷。使用聚合酶链反应（PCR）和核糖体 RNA（rRNA）基因内转录间隔区（ITS）序列分析鉴定和基因分型肠微孢子虫。肠微孢子虫的平均流行率为 8.72%（15/172），各村庄的流行率范围为 3.85%至 13.89%。肠微孢子虫感染与所研究的任何危险因素均无关。鉴定出 6 种基因型，包括 2 种已知基因型秘鲁 6 型（n=10）和 D 型（n=1）和 4 种新型基因型（MMR23、MMR25、MMR86 和 MMR87）（各 1 种），感染秘鲁 6 型的 2 人来自同一家庭。基于肠微孢子虫 ITS 序列的邻接法系统发育分析表明，所有 6 种基因型均聚为 1 组。
结论
这是缅甸首次对人类肠微孢子虫进行鉴定和基因分型。感染秘鲁 6 型的 2 人来自同一家庭，本研究获得的所有 6 种基因型均属于人畜共患病组 1，表明存在人为和人畜共患病传播的可能性。本研究数据表明，从医学角度进行流行病学控制和预防非常重要。
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