从人类脑组织中分离出博尔纳病病毒。
Isolation of Borna disease virus from human brain tissue.
作者信息
Nakamura Y, Takahashi H, Shoya Y, Nakaya T, Watanabe M, Tomonaga K, Iwahashi K, Ameno K, Momiyama N, Taniyama H, Sata T, Kurata T, de la Torre J C, Ikuta K
机构信息
Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060-0815, Japan.
出版信息
J Virol. 2000 May;74(10):4601-11. doi: 10.1128/jvi.74.10.4601-4611.2000.
Serological and molecular epidemiological studies indicate that Borna disease virus (BDV) can infect humans and is possibly associated with certain neuropsychiatric disorders. We examined brain tissue collected at autopsy from four schizophrenic patients and two healthy controls for the presence of BDV markers in 12 different brain regions. BDV RNA and antigen was detected in four brain regions of a BDV-seropositive schizophrenic patient (P2) with a very recent (2 years) onset of disease. BDV markers exhibited a regionally localized distribution. BDV RNA was found in newborn Mongolian gerbils intracranially inoculated with homogenates from BDV-positive brain regions of P2. Human oligodendroglia (OL) cells inoculated with brain homogenates from BDV-positive gerbils allowed propagation and isolation of BDVHuP2br, a human brain-derived BDV. Virus isolation was also possible by transfection of Vero cells with ribonucleoprotein complexes prepared from BDV-positive human and gerbil brain tissues. BDVHuP2br was genetically closely related to but distinct from previously reported human- and animal-derived BDV sequences.
血清学和分子流行病学研究表明，博尔纳病病毒（BDV）可感染人类，并可能与某些神经精神疾病有关。我们检测了4例精神分裂症患者和2例健康对照者尸检时采集的脑组织，以检测12个不同脑区中BDV标志物的存在情况。在一名疾病发病非常近期（2年）的BDV血清学阳性精神分裂症患者（P2）的4个脑区中检测到了BDV RNA和抗原。BDV标志物呈现出区域局限性分布。在颅内接种了来自P2的BDV阳性脑区匀浆的新生蒙古沙鼠中发现了BDV RNA。用人少突胶质细胞（OL）接种来自BDV阳性沙鼠的脑匀浆，可使源自人脑的BDV BDVHuP2br增殖并分离出来。用从BDV阳性的人和沙鼠脑组织制备的核糖核蛋白复合物转染Vero细胞，也能够实现病毒分离。BDVHuP2br在基因上与先前报道的源自人和动物的BDV序列密切相关，但又有所不同。
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