Ferreira J C, Panek A D, de Araujo P S
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
Can J Microbiol. 2000 Apr;46(4):383-6. doi: 10.1139/w99-136.
Maltose transport and maltase activities were inactivated during sporulation of a MAL constitutive yeast strain harboring different MAL loci. Both activities were reduced to almost zero after 5 h of incubation in sporulation medium. The inactivation of maltase and maltose permease seems to be related to optimal sporulation conditions such as a suitable supply of oxygen and cell concentration in the sporulating cultures, and occurs in the fully derepressed conditions of incubation in the sporulation acetate medium. The inactivation of maltase and maltose permease under sporulation conditions in MAL constitutive strains suggests an alternative mechanism for the regulation of the MAL gene expression during the sporulation process.
在携带不同MAL基因座的MAL组成型酵母菌株的孢子形成过程中，麦芽糖转运和麦芽糖酶活性被灭活。在孢子形成培养基中孵育5小时后，这两种活性几乎降至零。麦芽糖酶和麦芽糖通透酶的失活似乎与最佳孢子形成条件有关，如孢子形成培养物中合适的氧气供应和细胞浓度，并且发生在孢子形成醋酸盐培养基中完全去阻遏的孵育条件下。MAL组成型菌株在孢子形成条件下麦芽糖酶和麦芽糖通透酶的失活表明在孢子形成过程中MAL基因表达调控的另一种机制。
