利用不对称聚合酶链反应结合荧光偏振技术快速检测嗜肺军团菌基因。

Rapid detection of the gene of Legionella pneumophila using the fluorescence polarization with the asymmetric PCR.

作者信息

Fujii T, Ohta M, Kono M, Hoshina S, Fukuhara K, Tsuruoka M

机构信息

Towa Kagaku Co. Ltd., Hiroshima, Japan.

出版信息

Nucleic Acids Symp Ser. 1999(42):59-60. doi: 10.1093/nass/42.1.59.

DOI:10.1093/nass/42.1.59

PMID:10780378

Abstract

We attempted the rapid detection method of Legionella pneumophila by the asymmetric PCR and the fluorescence polarization. Eleven extracted DNAs from L. pneumophila serogroup 1 to approximately 6, L. bozemanii, L. dumoffii, L. gormanii, L. micdadei, and Pseudomonas aeruginosa were amplified by asymmetric PCR, and the polarization of those products were measured. Only the polarization of L. pneumophila serogroup 1 to approximately 6 rose within a few minutes after the beginning of measurement. The sensitivity to L. pneumophila using this method was 10(3) cells.

摘要

我们尝试通过不对称聚合酶链反应（PCR）和荧光偏振法对嗜肺军团菌进行快速检测。从嗜肺军团菌血清型1至血清型6、博兹曼军团菌、杜莫夫军团菌、戈尔曼军团菌、米德戴军团菌和铜绿假单胞菌中提取的11份DNA，通过不对称PCR进行扩增，并测量这些产物的偏振情况。仅嗜肺军团菌血清型1至血清型6的偏振情况在测量开始后的几分钟内升高。使用该方法对嗜肺军团菌的检测灵敏度为10³个细胞。