[通过聚合酶链反应法对从冷却塔水中分离出的嗜肺军团菌菌株进行鉴定]

[Identification of Legionella pneumophila strains by polymerase chain reaction method which isolated from cooling tower water].

作者信息

Koide M, Saito A, Ishimine T, Yamashiro Y, Higa F, Fukuhara H, Kawakami K, Kusano N

机构信息

First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus.

出版信息

Kansenshogaku Zasshi. 1995 Jan;69(1):79-84. doi: 10.11150/kansenshogakuzasshi1970.69.79.

DOI:10.11150/kansenshogakuzasshi1970.69.79

PMID:7751740

Abstract

We studied the polymerase chain reaction (PCR) method with 5 strains of Legionella pneumophila serogroup 4, 7 strains of L. pneumophila serogroup 5, 7 strains of L. pneumophila UT and 15 strains of Legionella like organisms (LLO). We used mip-primers reported by Mahbubani et al and LEG-primers reported by Starnbach et al. Positive PCR results were obtained by all strains of L. pneumophila serogroup 4, L. pneamophila serogroup 5, L. pneumophila UT and 11 strains of LLO using mip-primers. Using LEG-primers, positive results were obtained by 2, 6, 5 and 8 strains respectively. All strains that showed positive results using LEG-primer PCR, were contained by the strains that showed positive results using mip-primer PCR. Conversely, there were some strains that were positive in mip-primer PCR and negative in LEG-primer simultaneously. Therefore, mip-primers is recommended to detect L. pneumophila in clinical specimens by PCR method.

摘要

我们用4株嗜肺军团菌血清型4、7株嗜肺军团菌血清型5、7株嗜肺军团菌UT和15株类军团菌（LLO）研究了聚合酶链反应（PCR）方法。我们使用了Mahbubani等人报道的mip引物和Starnbach等人报道的LEG引物。使用mip引物时，嗜肺军团菌血清型4、嗜肺军团菌血清型5、嗜肺军团菌UT的所有菌株以及11株LLO均获得了PCR阳性结果。使用LEG引物时，分别有2株、6株、5株和8株获得阳性结果。所有使用LEG引物PCR呈阳性的菌株，均包含在使用mip引物PCR呈阳性的菌株中。相反，有一些菌株在mip引物PCR中呈阳性而在LEG引物PCR中同时呈阴性。因此，推荐使用mip引物通过PCR方法检测临床标本中的嗜肺军团菌。