Chatake T, Ono A, Ueno Y, Matsuda A, Takénaka A
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symp Ser. 1999(42):121-2. doi: 10.1093/nass/42.1.121.
In order to investigate mutation mechanism with oxyamine, two DNA dodecamers containing 2'-deoxy-N6-methoxyadenosine have been synthesized and their crystal structures have been determined. The dodecamers are associated in B form duplexes. The methoxy groups attached to the adenine bases do not affect the DNA conformation significantly. Electron densities clearly show that N6-methoxyadenine moiety forms Watson-Crick type pairing with both, thymine and cytosine bases. Such two faces in pairing are the origin of pyrimidine transition mutation.
为了研究羟胺的突变机制，已合成了两个含有2'-脱氧-N6-甲氧基腺苷的DNA十二聚体，并测定了它们的晶体结构。这些十二聚体以B型双链体形式存在。连接在腺嘌呤碱基上的甲氧基对DNA构象没有显著影响。电子密度清楚地表明，N6-甲氧基腺嘌呤部分与胸腺嘧啶和胞嘧啶碱基都形成沃森-克里克型配对。这种配对中的两个面是嘧啶转换突变的起源。
