含2'-脱氧-N6-甲氧基腺苷的DNA十二聚体的X射线分析
X-ray analysis of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine.
作者信息
Chatake T, Toyoda T, Tsunoda M, Sunami T, Ono A, Ueno Y, Matsuda A, Takenaka A
机构信息
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):97-8.
Oxyamines have been known as a mutagen which attacks amino groups of DNA bases. It is expected that the modified adenine derivative has a tautomer which can form a stable base pair not only with thymine but also with cytosine. For establishing such tautomerization mutagenesis, we have solved a crystal structure of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine. It is shown that the N6-methoxyadenine takes a imino form to form a Watson-Crick type pairing with cytosine in the DNA duplex.
氧胺类物质一直被认为是一种能攻击DNA碱基氨基的诱变剂。预计这种修饰后的腺嘌呤衍生物有一种互变异构体，它不仅能与胸腺嘧啶形成稳定的碱基对，还能与胞嘧啶形成稳定的碱基对。为了证实这种互变异构诱变作用，我们解析了含有2'-脱氧-N6-甲氧基腺苷的DNA十二聚体的晶体结构。结果表明，在DNA双链体中，N6-甲氧基腺嘌呤呈亚氨基形式，与胞嘧啶形成沃森-克里克型配对。
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