Park W S, Miyano-Kurosaki N, Abe T, Takai K, Takaku H
Department of Industrial, Chiba Institute of Technology, Japan.
Nucleic Acids Symp Ser. 1999(42):225-6. doi: 10.1093/nass/42.1.225.
We have designed a new class of oligonucleotides, "dumbbell RNA/DNA chimeric phosphodiesters", containing two alkyl loop structures with RNA/DNA base pairs (sense (RNA) and antisense (DNA) in the double helical stem. The reaction of nicked (NDRDON) and circular (CDRDON) dumbbell RNA/DNA chimeric oligonucleotides with RNaseH gave the corresponding antisense phosphodiester oligonucleotide together with the sense RNA cleavage products. The liberated antisense phosphodiester oligodeoxynucleotide was bound to the target 35mer RNA, which gave 35mer RNA cleavage products by treatment with RNaseH. The circular dumbbell RNA/DNA chimeric oligonucleotide showed more nuclease resistance than the linear antisense phosphodiester oligodeoxynucleotide(anti-ODN) and the nicked dumbbell RNA/DNA chimeric oligonucleotide.
我们设计了一类新型的寡核苷酸，即“哑铃状RNA/DNA嵌合磷酸二酯”，其在双螺旋茎中包含两个带有RNA/DNA碱基对的烷基环结构（有义链（RNA）和反义链（DNA））。带切口的（NDRDON）和环状的（CDRDON）哑铃状RNA/DNA嵌合寡核苷酸与RNaseH反应，生成了相应的反义磷酸二酯寡核苷酸以及有义链RNA的切割产物。释放出的反义磷酸二酯寡脱氧核苷酸与目标35聚体RNA结合，经RNaseH处理后产生35聚体RNA的切割产物。环状哑铃状RNA/DNA嵌合寡核苷酸比线性反义磷酸二酯寡脱氧核苷酸（反义ODN）和带切口的哑铃状RNA/DNA嵌合寡核苷酸表现出更强的核酸酶抗性。
