环状哑铃状RNA-DNA嵌合寡核苷酸的合成及其抗甲型流感病毒活性

Synthesis and anti-influenza virus-A activity of circular dumbbell RNA DNA chimeric oligonucleotides.

作者信息

Abe T, Hatta T, Yamakawa H, Takai K, Yokota T, Takaku H

机构信息

Department of Industrial Chemistry, Chiba Institute of Technology, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):219-20.

PMID:9586078

Abstract

We have designed a new type of antisense oligonucleotide, containing two hairpin loop structures with RNA/DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem (nicked and circular dumbbell DNA/RNA chimeric oligonucleotides). The reaction of the nicked and circular dumbbell DNA/RNA chimeric oligonucleotides with RNase H gave the corresponding anti-DNA together with the sense RNA cleavage products. These oligonucleotides were more resistant to exonuclease attack. We also describe the anti-Fluv activities of circular dumbbell DNA/RNA chimeric oligonucleotides.

摘要

我们设计了一种新型反义寡核苷酸，其在双螺旋茎中含有两个带有RNA/DNA碱基对（正义链（RNA）和反义链（DNA））的发夹环结构（带切口的环状哑铃状DNA/RNA嵌合寡核苷酸）。带切口的环状哑铃状DNA/RNA嵌合寡核苷酸与核糖核酸酶H反应产生了相应的抗DNA以及正义链RNA的切割产物。这些寡核苷酸对外切核酸酶的攻击更具抗性。我们还描述了环状哑铃状DNA/RNA嵌合寡核苷酸的抗流感病毒活性。