Yamakawa H, Hosono K, Ishibashi T, Takai K, Takaku H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Nucleic Acids Symp Ser. 1995(34):133-4.
We have designed a new type of antisense oligodeoxyribonucleotide. These oligonucleotides form two hairpin loop structures with RNA-DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem. The nicked and circular dumbbell RNA/DNA chimeric oligonucleotides are molecules, with or without free ends, that are more resistant to exonuclease attack. Of particular interest, antisense DNA is liberated by RNase H treatment of the dumbbell RNA/DNA chimeric oligonucleotides.
我们设计了一种新型反义寡脱氧核糖核苷酸。这些寡核苷酸在双螺旋茎中形成两个具有RNA-DNA碱基对（有义链（RNA）和反义链（DNA））的发夹环结构。带切口的环状哑铃状RNA/DNA嵌合寡核苷酸是有或没有自由末端的分子，它们对外切核酸酶的攻击更具抗性。特别值得关注的是，通过对哑铃状RNA/DNA嵌合寡核苷酸进行RNase H处理可释放反义DNA。
