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威胁邮件与法医学:经DFO处理后从证据物品中进行DNA分型

Threat mail and forensic science: DNA profiling from items of evidence after treatment with DFO.

作者信息

Zamir A, Oz C, Geller B

机构信息

Forensic Biology Laboratory, Division of Identification and Forensic Science, Israel Police National Headquarters, Jerusalem, Israel.

出版信息

J Forensic Sci. 2000 Mar;45(2):445-6.

PMID:10782971
Abstract

Two cases of threatening letters with their accompanying envelopes were received to the Division of Forensic Identification unit of the Israel Police. The envelopes, including the stamps, and the letters were initially examined for latent fingerprints by the DFO reagent, known to cause degradation of DNA. Although no latent fingerprints could be visualized on any of the items, the biology laboratory using organic DNA extraction, was successful in defining genetic profiles from all the items employing six STR loci, even after treatment with DFO. In a controlled experiment, a known donor attached a stamp, by licking, to an envelope. This item was treated with DFO and then profiled using STR loci. The results showed that previous DFO treatment on the control stamp before DNA analysis had no negative effects on obtaining the DNA profile of the known donor using STR loci.

摘要

以色列警方法医鉴定部门收到了两封附有信封的恐吓信。包括邮票在内的信封和信件最初使用DFO试剂进行了潜在指纹检查,已知该试剂会导致DNA降解。尽管在任何物品上都未发现潜在指纹,但生物学实验室采用有机DNA提取方法,即使在经过DFO处理后,仍成功地从所有物品中利用六个STR基因座确定了基因图谱。在一项对照实验中,一名已知捐赠者通过舔舐在一个信封上贴上了邮票。该物品经过DFO处理,然后使用STR基因座进行分析。结果表明,在DNA分析之前对对照邮票进行的DFO处理对使用STR基因座获取已知捐赠者的DNA图谱没有负面影响。

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