Xu J, Locascio L, Gaitan M, Lee CS
Department of Chemistry and Biochemistry, University of Maryland, College Park 20742, USA.
Anal Chem. 2000 Apr 15;72(8):1930-3. doi: 10.1021/ac991216q.
A new plastic imprinting method using a silicon template is demonstrated. This new approach obviates the necessity of heating the plastic substrate during the stamping process, thus improving the device yield from approximately 10 devices to above 100 devices per template. The dimensions of the imprinted microchannels were found to be very reproducible, with variations of less than 2%. The channel depths were dependent on the pressures applied and the materials used. Rather than bonding the open channels with another piece of plastic, a flexible and adhesive poly(dimethylsiloxane) film is used to seal the microchannels, which offers many advantages. As an application, isoelectric focusing of green fluorescence protein on these plastic microfluidic devices is illustrated.
展示了一种使用硅模板的新型塑料压印方法。这种新方法消除了在冲压过程中加热塑料基板的必要性，从而将每个模板的器件产量从大约10个提高到100个以上。发现压印微通道的尺寸具有很高的可重复性，变化小于2%。通道深度取决于所施加的压力和所使用的材料。不是用另一片塑料粘结开放通道，而是使用柔性且有粘性的聚二甲基硅氧烷薄膜来密封微通道，这具有许多优点。作为一种应用，展示了绿色荧光蛋白在这些塑料微流控器件上的等电聚焦。
