Chida T, Okamura N, Ohtani K, Yoshida Y, Arakawa E, Watanabe H
Department of Clinical Microbiology and Immunology, School of Allied Health Sciences, Tokyo Medical and Dental University, Japan.
Microbiol Immunol. 2000;44(3):161-72. doi: 10.1111/j.1348-0421.2000.tb02478.x.
We cloned and determined the sequence of a DNA region of approximately 15-kb containing the cluster of genes required for O17 antigen expression in the Escherichia coli K-12 strain from the chromosome of Plesiomonas shigelloides serotype O17:H2 strain. The sequencing analysis revealed that the minimum essential region of the P. shigelloides O17 antigen gene cluster had a size of approximately 11.5-kb and contained 9 contiguous open reading frames (ORFs), which were almost identical to the corresponding ORFs of Shigella sonnei form I antigen gene region, except for IS630 sequence, at the DNA as well as amino acid levels. The putative function of most of the ORFs could be determined on the basis of amino acid sequence similarities and characteristics. In addition, the G+C content of the P. shigelloides O17 antigen genes was lower than that of the chromosomal DNA of P. shigelloides and S. sonnei, suggesting that both P. shigelloides O17 and S. sonnei form I antigen genes had been derived from the same origin with a low G+C content.
我们从宋内志贺氏菌血清型O17:H2菌株的染色体中克隆并测定了大肠杆菌K-12菌株中表达O17抗原所需基因簇的一段约15kb的DNA区域的序列。测序分析表明，宋内志贺氏菌O17抗原基因簇的最小必需区域大小约为11.5kb，包含9个连续的开放阅读框（ORF），这些开放阅读框在DNA和氨基酸水平上，除了IS630序列外，与宋内志贺氏菌I型抗原基因区域的相应ORF几乎相同。大多数ORF的推定功能可根据氨基酸序列的相似性和特征来确定。此外，宋内志贺氏菌O17抗原基因的G+C含量低于宋内志贺氏菌和宋内志贺氏菌的染色体DNA，这表明宋内志贺氏菌O17和宋内志贺氏菌I型抗原基因均来自具有低G+C含量的同一来源。
