Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University, Tianjin 300457, P.R. China.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, P.R. China.
J Microbiol Biotechnol. 2021 Apr 28;31(4):520-528. doi: 10.4014/jmb.2010.10008.
, a member of the family , is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. contains 102 Oantigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between and . Only O38 possesses the and genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using and showed that both genes are diversified. Among the nine new serogroups, eight use genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.
是弧菌科的一种革兰氏阴性、杆状、兼性厌氧细菌，具有鞭毛。已从淡水、地表水和许多野生动物和家养动物等来源中分离出来。包含 102 个 O 抗原和 51 个 H 抗原。O 抗原基因簇的多样性相对了解较少。除了其他实验室报道的 O1 和 O17 以及我们之前报道的 12 个 O 血清群（O2、O10、O12、O23、O25、O26、O32、O33、O34、O66、O75 和 O76）外，在本研究中，还测序并注释了 9 个新的 血清群（O8、O17、O18、O37、O38、O39、O44、O45 和 O61）。这 9 个群的 O 抗原基因位于染色体上的 和 之间。只有 O38 具有通过 ATP 结合盒（ABC）转运蛋白途径合成和转运 O 抗原的 和 基因；其他 8 个使用 Wzx/Wzy 途径。使用 和 进行的系统发育分析表明，这两个基因都具有多样性。在这 9 个新的 血清群中，有 8 个使用 基因作为靶标。此外，我们开发了一种 O 抗原特异性 PCR 检测方法，可检测这 9 个不同的血清群，它们之间没有交叉反应。
