Xi Daoyi, Wang Xiaochen, Ning Kexin, Liu Qian, Jing Fuyi, Guo Xi, Cao Boyang
Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University, Tianjin, China.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China.
Front Microbiol. 2019 Apr 10;10:741. doi: 10.3389/fmicb.2019.00741. eCollection 2019.
is a Gram-negative, flagellated, rod-shaped, ubiquitous, and facultative anaerobic bacterium. It has been isolated from various sources, such as freshwater, surface water, and many wild and domestic animals. is associated with diarrheal diseases of acute secretory gastroenteritis, an invasive shigellosis-like disease, and a cholera-like illness in humans. At present, 102 somatic antigens and 51 flagellar antigens of have been recognized; however, very little is known about variations of O-antigens among species. In this study, 12 O-antigen gene clusters of , O2H1a1c (G5877), O10H41 (G5892), O12H35 (G5890), O23H1a1c (G5263), O25H3 (G5879), O26H1a1c (G5889), O32H37 (G5880), O33H38 (G5881), O34H34 (G5882), O66H3 (G5270), O75H34 (G5885), and O76H39 (G5886), were sequenced and analyzed. The genes that control O-antigen synthesis are present as chromosomal gene clusters that maps between and , and most of the synthesis and translocation of OPS (O-specific polysaccharide) belongs to Wzx/Wzy pathway with the exception of O12, O25, and O66, which use the ATP-binding cassette (ABC) transporter pathway. Phylogenetic analysis of and show that the and genes are specific to individual O-antigens and can be used as targets in molecular typing. Based on the sequence data, an O-antigen specific suspension array that detects 12 distinct OPS' has been developed. This is the first report to catalog the genetic features of O-antigen variations and develop a suspension array for the molecular typing. The method has several advantages over traditional bacteriophage and serum agglutination methods and lays the foundation for easier identification and detection of additional O-antigen in the future.
是一种革兰氏阴性、有鞭毛、杆状、无处不在的兼性厌氧菌。它已从各种来源分离出来，如淡水、地表水以及许多野生动物和家畜。它与人类的急性分泌性肠胃炎腹泻病、一种侵袭性志贺氏菌样疾病以及一种霍乱样疾病有关。目前，已识别出该菌的102种菌体抗原和51种鞭毛抗原；然而，关于该菌不同物种间O抗原的变异情况知之甚少。在本研究中，对该菌的12个O抗原基因簇，即O2H1a1c（G5877）、O10H41（G5892）、O12H35（G5890）、O23H1a1c（G5263）、O25H3（G5879）、O26H1a1c（G5889）、O32H37（G5880）、O33H38（G5881）、O34H34（G5882）、O66H3（G5270）、O75H34（G5885）和O76H39（G5886）进行了测序和分析。控制O抗原合成的基因以染色体基因簇的形式存在，定位于某两个基因之间，除了O12、O25和O66使用ATP结合盒（ABC）转运途径外，大多数OPS（O特异性多糖）的合成和转运属于Wzx/Wzy途径。对该菌和其他相关菌的系统发育分析表明，该菌的某些基因对个别O抗原具有特异性，可作为分子分型的靶点。基于序列数据，开发了一种可检测12种不同OPS的O抗原特异性悬浮阵列。这是第一份对该菌O抗原变异的遗传特征进行编目并开发用于分子分型的悬浮阵列的报告。该方法相对于传统的噬菌体和血清凝集方法具有多个优点，并为未来更轻松地鉴定和检测其他O抗原奠定了基础。
