Kim S H, Song K J, Shin Y K, Kim J H, Choi S M, Park K S, Baek L J, Lee Y J, Song J W
Department of Microbiology, Institute for Viral Diseases, College of Medicine, Korea University, Seoul.
Microbiol Immunol. 2000;44(3):173-7. doi: 10.1111/j.1348-0421.2000.tb02479.x.
Viral RNAs extracted from fifteen mumps virus isolated from throat swab, saliva, blood, urine or CSF during mumps epidemics between 1997-1998 in Korea were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and compared by nucleotide sequencing of the small hydrophobic (SH) gene. The deduced amino acid sequences of the SH gene were aligned with the published sequences of mumps virus isolated in different geographic areas. A comparison of the SH gene of mumps viruses in Korea indicated 96.2-100% and 91.2-100% similarity at the nucleotide and amino acid levels, respectively. Phylogenetic analysis, using the neighbor-joining method, showed that Korean mumps virus strains formed a genetically distinct monophyletic group from previously reported genotypes based on the 315-bp length nucleotide and 57 deduced amino acid sequences of the SH gene, and possibly be designated as a new genotype (I).
从1997年至1998年韩国腮腺炎流行期间从咽喉拭子、唾液、血液、尿液或脑脊液中分离出的15株腮腺炎病毒中提取的病毒RNA，通过逆转录聚合酶链反应（RT-PCR）进行扩增，并通过小疏水（SH）基因的核苷酸测序进行比较。将SH基因推导的氨基酸序列与在不同地理区域分离的腮腺炎病毒的已发表序列进行比对。韩国腮腺炎病毒SH基因的比较表明，在核苷酸和氨基酸水平上的相似性分别为96.2%-100%和91.2%-100%。使用邻接法进行的系统发育分析表明，基于SH基因315 bp长度的核苷酸和57个推导的氨基酸序列，韩国腮腺炎病毒株形成了一个与先前报道的基因型在遗传上不同的单系群，可能被指定为一个新的基因型（I）。
