Afzal M A, Buchanan J, Dias J A, Cordeiro M, Bentley M L, Shorrock C A, Minor P D
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Herts, United Kingdom.
J Med Virol. 1997 Aug;52(4):349-53. doi: 10.1002/(sici)1096-9071(199708)52:4<349::aid-jmv1>3.0.co;2-1.
Clinical specimens collected during an outbreak of mumps were characterised by RT-PCR, nested PCR, and nucleotide sequencing. Mumps virus was positively identified in 12/21(57%) saliva, 9/21(43%), throat and 1/33(3%) urine specimens and further sequence comparison revealed that at least six strains of viruses, which differed from 0-9.43% at the nucleotide levels, were cocirculating during the epidemic. However, phylogenetic analysis showed that these viruses grouped with two previously identified lineages which were mostly composed of other European mumps virus isolates.
在腮腺炎暴发期间收集的临床标本通过逆转录聚合酶链反应（RT-PCR）、巢式PCR和核苷酸测序进行了特征分析。在12/21（57%）的唾液标本、9/21（43%）的咽喉标本和1/33（3%）的尿液标本中阳性鉴定出腮腺炎病毒，进一步的序列比较显示，在疫情期间至少有六种病毒株在共同传播，它们在核苷酸水平上的差异为0-9.43%。然而，系统发育分析表明，这些病毒与之前鉴定的两个谱系归为一组，这两个谱系主要由其他欧洲腮腺炎病毒分离株组成。
