在现场研究中收集的唾液中检测人疱疹病毒6型和7型的灵敏方法。
Sensitive method for detection of human herpesviruses 6 and 7 in saliva collected in field studies.
作者信息
Zerr D M, Huang M L, Corey L, Erickson M, Parker H L, Frenkel L M
机构信息
Department of Pediatrics, University of Washington, Seattle, USA.
出版信息
J Clin Microbiol. 2000 May;38(5):1981-3. doi: 10.1128/JCM.38.5.1981-1983.2000.
Abstract
To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.
摘要
为便于对婴儿人疱疹病毒6型和7型的流行病学及自然史进行研究，开发了一种收集和定量这些病毒DNA的实用方法。使用小条滤纸收集唾液，并通过实时定量荧光探针PCR检测法检测病毒。即使标本长时间干燥后，该方法的敏感性和特异性与我们传统的唾液收集和检测方法相比仍具有优势。
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