Shanehsazzadeh Mehrnaz, Rad Javad Sharifi-, Pourazar Abbasali, Behbahani Mandana
Department of Medicinal Biology, Zist Partak Institute, Academic Center for Education and Research, Isfahan, Iran.
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran ; Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
Med Arch. 2014 Aug;68(4):276-8. doi: 10.5455/medarh.2014.68.276-278. Epub 2014 Jul 31.
The previous studies showed that herpes human virus-6 (HHV-6) and HHV-7 exist in salivary glands. One of the important areas in oral and maxillofacial pathology field is tumors of the salivary glands. In this study, to declare the major sites of persistent infection with HHV-6 and HHV-7, the existence of HHV-6 and HHV-7 genomes in formalin-fixed paraffin embedded tissue samples of salivary gland tumors.
This analytical study was performed in 60 paraffin blocks samples of malignant and benign neoplasms of both major and minor salivary glands. This study performed with highly sensitive real time PCR method.
Among 60 paraffin blocks salivary gland tumors with equal chances of presence of the HHV-7 and HHV-6 in the samples, 34% were positive for both HHV-7 and HHV-6 while 47.2% were only positive for HHV-7, 18.9% samples were positive for HHV-6. A relationship was noticed between HHV-7 and HHV-6 genomes.
In conclusion, this study showed no relation between virus and diseases with P=0.953. Also it could be inferred that there is a relationship between HHV-6 and 7 in salivary glands neoplasms.
先前的研究表明，人类疱疹病毒6型（HHV-6）和HHV-7存在于唾液腺中。口腔颌面病理学领域的一个重要研究方向是唾液腺肿瘤。在本研究中，为了明确HHV-6和HHV-7持续感染的主要部位，对唾液腺肿瘤的福尔马林固定石蜡包埋组织样本中HHV-6和HHV-7基因组的存在情况进行了研究。
本分析研究对60个来自大唾液腺和小唾液腺良恶性肿瘤的石蜡块样本进行。本研究采用高灵敏度实时聚合酶链反应方法。
在60个石蜡块唾液腺肿瘤样本中，HHV-7和HHV-6存在几率相同，其中34%的样本HHV-7和HHV-6均呈阳性，47.2%的样本仅HHV-7呈阳性，18.9%的样本HHV-6呈阳性。注意到HHV-7和HHV-6基因组之间存在关联。
总之，本研究表明病毒与疾病之间无关联，P = 0.953。还可以推断，在唾液腺肿瘤中HHV-6和HHV-7之间存在关联。
