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本文引用的文献

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Comparison of genetic variability at multiple loci across the genomes of the major subtypes of Kaposi's sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame K15 alleles at the right-hand end.卡波西肉瘤相关疱疹病毒主要亚型基因组中多个位点的遗传变异性比较揭示了重组证据以及右手端存在两种不同类型的开放阅读框K15等位基因。
J Virol. 1999 Aug;73(8):6646-60. doi: 10.1128/JVI.73.8.6646-6660.1999.
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Quantitation of 8 human herpesviruses in peripheral blood of human immunodeficiency virus-infected patients and healthy blood donors by polymerase chain reaction.
Am J Clin Pathol. 1999 May;111(5):655-9. doi: 10.1093/ajcp/111.5.655.
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Human herpesvirus 8 DNA load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of Kaposi's sarcoma and response to anticytomegalovirus therapy.人类免疫缺陷病毒感染受试者白细胞中的人类疱疹病毒8型DNA载量：与卡波西肉瘤的存在及抗巨细胞病毒治疗反应的相关性
Antimicrob Agents Chemother. 1999 Feb;43(2):377-80. doi: 10.1128/AAC.43.2.377.
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Quantitative RT-PCR: pitfalls and potential.定量逆转录聚合酶链反应：陷阱与潜力
Biotechniques. 1999 Jan;26(1):112-22, 124-5. doi: 10.2144/99261rv01.
5
Lymphoma cell lines: in vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas).淋巴瘤细胞系：用于研究HHV-8阳性原发性渗出性淋巴瘤（体腔淋巴瘤）的体外模型。
Leukemia. 1998 Oct;12(10):1507-17. doi: 10.1038/sj.leu.2401160.
6
Geographically distinct HHV-8 DNA sequences in Saudi Arabian Iatrogenic Kaposi's sarcoma lesions.沙特阿拉伯医源性卡波西肉瘤病变中地理上不同的HHV-8 DNA序列。
Am J Pathol. 1998 Sep;153(3):1001-4. doi: 10.1016/S0002-9440(10)65642-8.
7
Human herpesvirus 8 in Italian HIV-seronegative patients with Kaposi sarcoma.意大利HIV血清阴性卡波西肉瘤患者中的人类疱疹病毒8型
Arch Dermatol. 1998 Jun;134(6):695-9. doi: 10.1001/archderm.134.6.695.
8
Human herpesvirus 8 group B and C variants circulating in São Paulo, Brazil.在巴西圣保罗市传播的人类疱疹病毒8型B组和C组变体。
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9
Strain variability among Kaposi sarcoma-associated herpesvirus (human herpesvirus 8) genomes: evidence that a large cohort of United States AIDS patients may have been infected by a single common isolate.卡波西肉瘤相关疱疹病毒（人类疱疹病毒8型）基因组间的毒株变异性：美国一大群艾滋病患者可能受单一常见毒株感染的证据。
J Virol. 1997 Mar;71(3):2505-11. doi: 10.1128/JVI.71.3.2505-2511.1997.
10
Development of a quantitative competitive polymerase chain reaction for human herpesvirus 8.人疱疹病毒8型定量竞争性聚合酶链反应的开发
J Virol Methods. 1997 Feb;64(1):19-26. doi: 10.1016/s0166-0934(96)02139-8.

通过实时聚合酶链反应对游离和细胞相关的卡波西肉瘤相关疱疹病毒DNA进行定量分析。

Quantitation of cell-free and cell-associated Kaposi's sarcoma-associated herpesvirus DNA by real-time PCR.

作者信息

White I E, Campbell T B

机构信息

Division of Infectious Diseases, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado, USA.

出版信息

J Clin Microbiol. 2000 May;38(5):1992-5. doi: 10.1128/JCM.38.5.1992-1995.2000.

DOI:10.1128/JCM.38.5.1992-1995.2000
PMID:10790138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC86648/
Abstract

A real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 10(5) copies of KSHV DNA (r(2) > 0.99). The accuracy of DNA purification and quantitation was less than +/-0.4 log(10) copies for samples that contained from 10 to 10(5) copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0. 1 to 10(-5), and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 10(6) copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens.

摘要

对一种用于定量卡波西肉瘤相关疱疹病毒（KSHV或人类疱疹病毒8型）DNA的实时聚合酶链反应检测法进行了评估。线性动态范围为10至10(5)个KSHV DNA拷贝（r(2)>0.99）。对于含有10至10(5)个KSHV DNA拷贝的样本，DNA纯化和定量的准确度小于±0.4 log(10)拷贝。在0.1至10(-5)的一系列感染细胞频率范围内对细胞相关的KSHV DNA进行了定量，在100至10(6)拷贝/毫升的范围内对血浆中无细胞KSHV DNA进行了定量。实时聚合酶链反应为实验室和临床标本中无细胞和细胞相关KSHV DNA的定量提供了一种便捷方法。