Foreman K E, Alkan S, Krueger A E, Panella J R, Swinnen L J, Nickoloff B J
Department of Pathology, Cardinal Bernardin Cancer Center, Loyola University Medical Center, Maywood, Illinois, USA.
Am J Pathol. 1998 Sep;153(3):1001-4. doi: 10.1016/S0002-9440(10)65642-8.
A new member of the gamma-herpesvirus family, HHV-8 (also known as Kaposi's sarcoma (KS)-associated herpesvirus), has been linked to KS and body cavity-based lymphoma. Other members of this family, eg, Epstein-Barr virus, were originally thought to have only one strain, but subsequent analysis revealed different strains correlating to cellular patterns of infectivity and geographical location. To determine whether multiple strains of HHV-8 exist, we compared DNA sequences among KS and body cavity-based lymphoma-derived HHV-8 and examined differences in HHV-8 subgroups between American and Saudi Arabian iatrogenic KS patients. Samples were analyzed by polymerase chain reaction using multiple primer sets to five different open reading frames from HHV-8, and DNA sequencing was performed. HHV-8 DNA was present in all of our KS and body cavity-based lymphoma samples by polymerase chain reaction. HHV-8 DNA was detected in each body cavity-based lymphoma sample using a majority of the primers, whereas only two primer sets consistently amplified HHV-8 DNA derived from KS lesions. DNA sequencing within open reading frames 26 and 27 indicate the existence of at least three variants of HHV-8, with the majority of iatrogenic KS patients in Saudi Arabia containing unique nucleotide changes that may define a distinct, previously unidentified subgroup we term SA, whereas those from America were of Group A or B. Thus, although the sequencing data within open reading frames 26 and 27 did not permit discrimination between patients with lymphoma versus KS disease processes, HHV-8 derived from Saudi Arabian KS lesions were shown to have a distinct nucleotide sequence not seen in any of the other clinical samples examined.
γ-疱疹病毒家族的一个新成员，即人类疱疹病毒8型（HHV-8，也称为卡波西肉瘤相关疱疹病毒），已被证实与卡波西肉瘤（KS）和体腔淋巴瘤有关。该家族的其他成员，如爱泼斯坦-巴尔病毒，最初被认为只有一个毒株，但随后的分析显示，不同毒株与细胞感染模式和地理位置相关。为了确定HHV-8是否存在多个毒株，我们比较了KS和体腔淋巴瘤来源的HHV-8的DNA序列，并研究了美国和沙特医源性KS患者之间HHV-8亚组的差异。使用针对HHV-8五个不同开放阅读框的多组引物，通过聚合酶链反应对样本进行分析，并进行DNA测序。通过聚合酶链反应，我们所有的KS和体腔淋巴瘤样本中均检测到HHV-8 DNA。使用大多数引物在每个体腔淋巴瘤样本中均检测到HHV-8 DNA，而只有两组引物能持续扩增来自KS病变的HHV-8 DNA。开放阅读框26和27内的DNA测序表明至少存在三种HHV-8变体，沙特大多数医源性KS患者含有独特的核苷酸变化，这些变化可能定义了一个独特的、以前未鉴定的亚组，我们称之为SA亚组，而来自美国的患者属于A组或B组。因此，尽管开放阅读框26和27内的测序数据无法区分淋巴瘤患者和KS疾病患者，但沙特KS病变来源的HHV-8显示出一种独特的核苷酸序列，在其他任何检测的临床样本中均未发现。
