Bolzani G, Della Coletta R, Martelli Júnior H, Martelli Júnior H, Graner E
Department of Oral Pathology and Genetics, School of Dentistry of Piracicaba, UNICAMP, SP, Brazil.
J Periodontal Res. 2000 Feb;35(1):51-8. doi: 10.1034/j.1600-0765.2000.035001051.x.
Cyclosporin A (CyA) is a potent immunosuppressor used in organ transplantation and in the management of various autoimmune diseases. Gingival overgrowth is one of the side-effects of the CyA-treatment, affecting the attached gingiva of 25-81% of treated patients. To investigate the production and activity of matrix metalloproteinases (MMPs) in the CyA-induced gingival overgrowth, 2 well-documented models were utilized: the in vivo CyA-induced rat gingival overgrowth and primary cultures of human gingival fibroblasts treated with CyA. Our results obtained from the Western blot assays demonstrated clearly that the production of MMP-1 and MMP-3 was significantly inhibited by CyA at similar concentrations found in the serum of patients undergoing CyA-treatment. Moreover, the gelatinolytic activity of MMP-2 was also reduced both in cultured fibroblasts and in the rat CyA-induced gingival overgrowth. Taken together, the data presented here suggest that these inhibitory effects may contribute to the extracellular matrix (ECM) components accumulation in the CyA-induced gingival overgrowth.
环孢素A（CyA）是一种强效免疫抑制剂，用于器官移植和各种自身免疫性疾病的治疗。牙龈增生是CyA治疗的副作用之一，影响25%-81%接受治疗患者的附着龈。为了研究基质金属蛋白酶（MMPs）在CyA诱导的牙龈增生中的产生和活性，使用了2个充分记录的模型：体内CyA诱导的大鼠牙龈增生和用CyA处理的人牙龈成纤维细胞原代培养物。我们从蛋白质印迹分析中获得的结果清楚地表明，在接受CyA治疗患者血清中发现的相似浓度下，CyA显著抑制MMP-1和MMP-3的产生。此外，MMP-2的明胶酶活性在培养的成纤维细胞和大鼠CyA诱导的牙龈增生中也降低。综上所述，此处呈现的数据表明这些抑制作用可能导致CyA诱导的牙龈增生中细胞外基质（ECM）成分的积累。
