Huang Y, Yang J, Wang X B, Becker F F, Gascoyne P R
Department of Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
J Hematother Stem Cell Res. 1999 Oct;8(5):481-90. doi: 10.1089/152581699319939.
Dielectrophoretic field-flow-fractionation (DEP-FFF) was used to purge human breast cancer MDA-435 cells from hematopoietic CD34+ stem cells. An array of interdigitated microelectrodes lining the bottom surface of a thin chamber was used to generate dielectrophoretic forces that levitated the cell mixture in a fluid flow profile. CD34+ stem cells were levitated higher, were carried faster by the fluid flow, and exited the separation chamber earlier than the cancer cells. Using on-line flow cytometry, efficient separation of the cell mixture was observed in less than 12 min, and CD34+ stem cell fractions with a purity >99.2% were obtained. The method of DEP-FFF is potentially applicable to many biomedical cell separation problems, including microfluidic-scale diagnosis and preparative-scale purification of cell subpopulations.
介电泳场流分级法（DEP-FFF）被用于从造血CD34+干细胞中清除人乳腺癌MDA-435细胞。在一个薄腔室底面排列的一系列叉指状微电极被用于产生介电泳力，该力使细胞混合物悬浮在流体流动剖面中。CD34+干细胞悬浮得更高，被流体携带得更快，并且比癌细胞更早离开分离腔室。使用在线流式细胞术，在不到12分钟内观察到细胞混合物的高效分离，并且获得了纯度>99.2%的CD34+干细胞级分。DEP-FFF方法可能适用于许多生物医学细胞分离问题，包括微流体规模的诊断和细胞亚群的制备规模纯化。
