Cheng J, Sheldon E L, Wu L, Heller M J, O'Connell J P
Nanogen Inc., San Diego, California 92121, USA.
Anal Chem. 1998 Jun 1;70(11):2321-6. doi: 10.1021/ac971274g.
The separation and subsequent isolation of the metastatic human cervical carcinoma cell line (HeLa cells) from normal human peripheral blood cells has been achieved by exploiting their differential dielectric properties. The isolation process is carried out on a silicon chip containing a five-by-five array of microlocations. These microlocations contain underlying circular platinum electrodes with 80-micron diameters and center-to-center spacing of 200 microns. The surfaces of the electrodes and nonmetallized areas have been coated with a permeation layer to prevent the direct contact of cells with the electrode and also to minimize the nonspecific adhesion of the cells to the chip surface. An inhomogenous ac field is applied to the electrodes to create the conditions for dielectrophoretic separation of cells. Cell separation using dielectrophoresis as well as electronic lysis on a silicon chip would provide essential sample-processing steps which may be combined with a later multiplex electronic hybridization step in an integrated assay system.
通过利用转移性人类宫颈癌细胞系（HeLa细胞）与正常人外周血细胞的介电特性差异，实现了从正常人外周血细胞中分离并随后分离出该癌细胞系。分离过程在一个包含5×5微定位阵列的硅芯片上进行。这些微定位包含直径为80微米、中心间距为200微米的圆形铂底层电极。电极表面和未金属化区域已涂有渗透层，以防止细胞与电极直接接触，并尽量减少细胞与芯片表面的非特异性粘附。向电极施加非均匀交流电场，以创造细胞介电泳分离的条件。使用介电泳进行细胞分离以及在硅芯片上进行电子裂解将提供基本的样品处理步骤，这些步骤可与集成检测系统中后续的多重电子杂交步骤相结合。
