Yang J, Huang Y, Wang X B, Becker F F, Gascoyne P R
Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Biophys J. 2000 May;78(5):2680-9. doi: 10.1016/S0006-3495(00)76812-3.
The differential analysis of human leukocytes has many important biological and medical applications. In this work, dielectrophoretic field-flow-fractionation (DEP-FFF), a cell-separation technique that exploits the differences in the density and dielectric properties of cells, was used to separate the mixtures of the major human leukocyte subpopulations (T- and B-lymphocytes, monocytes, and granulocytes). The separation was conducted in a thin chamber equipped with an array of microfabricated interdigitated electrodes on the bottom surface, and the separation performance was characterized by on-line flow cytometry. To investigate optimal separation conditions for different leukocyte mixtures, elution fractograms at various DEP field frequencies were obtained for each leukocyte subtype. With appropriately chosen conditions, high separation performance was achieved in separating T- (or B-) lymphocytes from monocytes, T- (or B-) lymphocytes from granulocytes, and monocytes from granulocytes. DEP-FFF does not involve cell-labeling or cell-modification step, and provides a new approach to hematological analysis.
人类白细胞的差异分析具有许多重要的生物学和医学应用。在这项工作中，介电泳场流分离法（DEP-FFF），一种利用细胞密度和介电特性差异的细胞分离技术，被用于分离主要人类白细胞亚群（T淋巴细胞、B淋巴细胞、单核细胞和粒细胞）的混合物。分离在一个底部表面配备有微加工叉指电极阵列的薄腔室中进行，分离性能通过在线流式细胞术进行表征。为了研究不同白细胞混合物的最佳分离条件，针对每种白细胞亚型，在不同的DEP场频率下获得了洗脱分馏图。通过适当选择条件，在分离T（或B）淋巴细胞与单核细胞、T（或B）淋巴细胞与粒细胞以及单核细胞与粒细胞方面实现了高分离性能。DEP-FFF不涉及细胞标记或细胞修饰步骤，为血液学分析提供了一种新方法。
