Kato A C, Borstad L, Fraser M J, Denhardt D T
Nucleic Acids Res. 1974 Nov;1(11):1539-48. doi: 10.1093/nar/1.11.1539.
We have used the single-strand specific nuclease from Neurospora crassa and chromatography on methylated albumin-kieselguhr to purify and characterize repeated and self-complementary sequences from Escherichia coli DNA. Approximately 0.5% of the genome renatures spontaneously at zero time and another 2% renatures somewhat more rapidly than the total DNA. The early renaturing DNA has a base composition and a Tm similar to the total DNA and contains on the average 100 base pairs; the self-complementary DNA also has a base composition like E. coli but contains a mean of 170 base pairs. No evidence was obtained for the presence of a highly redundant sequence.
我们使用了来自粗糙脉孢菌的单链特异性核酸酶,并通过甲基化白蛋白-硅藻土柱层析,从大肠杆菌DNA中纯化和鉴定重复及自我互补序列。基因组中约0.5%在零时间自发复性,另外2%的复性速度比总DNA稍快。早期复性的DNA碱基组成和熔解温度与总DNA相似,平均含有100个碱基对;自我互补DNA的碱基组成也与大肠杆菌相似,但平均含有170个碱基对。未获得高度冗余序列存在的证据。