Shida T, Iwasaki H, Shinagawa H, Kyogoku Y
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Nucleic Acids Symp Ser. 1995(34):5-6.
Sixteen bimobile four-way junction DNAs were designed to clarify the consensus sequence at the resolution site by the E. coli RuvC protein. The consensus sequence is 5'-T decreases X-3' (X: A, G, C, and T) without exception, but the sequences at the cleaved sites are not necessarily symmetric. The presence of symmetrically located two AT base-pairs at the junction is the minimal requirement for resolution.
设计了16种双活动四向连接DNA，以阐明大肠杆菌RuvC蛋白在切割位点的共有序列。共有序列毫无例外都是5'-T↓X-3'（X：A、G、C和T），但切割位点处的序列不一定是对称的。连接处对称定位的两个AT碱基对的存在是进行切割的最低要求。
