Sundaram P V
Nucleic Acids Res. 1974 Nov;1(11):1587-99. doi: 10.1093/nar/1.11.1587.
Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and cofactors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.
本文描述了核苷酸,特别是衍生化核苷酸、多核苷酸和辅因子与不溶性聚合物共价偶联的一些通用方法。必要时,个别方法还包含有关酶与相同聚合物结合的一些信息,以作为偶联方法效率的指南。