通过将溴化氰活化的纤维素与5'-氨基末端寡核苷酸和聚（pdT）偶联来制备与固相支持物相连的聚脱氧核苷酸。
Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.
作者信息
Clerici L, Campagnari F, de Rooij J F, van Boom J H
出版信息
Nucleic Acids Res. 1979 Jan;6(1):247-58. doi: 10.1093/nar/6.1.247.
Abstract
Synthetically-prepared 5'-NH2-dT(pdT)n oligomers (66,n=4 or 7) were immobilized on cyanogen bromide activated cellulose. The influence of temperature, pH, and ionic strength on the rate of the coupling process was studied. The oligomer 5'-NH2-DT(pdT)8 could be elongated enzymatically to the polymers 5'-NH2-dT(pdT)n (n=20, 51 and 84), which could be immobilized on cellulose. The cellulose-NH-dT(pdT)84 polymer thus obtained could be assembled to a new solid-state polymer e.g. poly(dA)290 . poly(/3H/dT)200, poly(dT)85-cellulose which, in turn, was a very convenient substrate for assaying DNA-ligase.
摘要
合成制备的5'-NH2-dT(pdT)n寡聚物（66，n = 4或7）被固定在溴化氰活化的纤维素上。研究了温度、pH值和离子强度对偶联过程速率的影响。寡聚物5'-NH2-DT(pdT)8可以通过酶促反应延长为聚合物5'-NH2-dT(pdT)n（n = 20、51和84），这些聚合物可以固定在纤维素上。由此得到的纤维素-NH-dT(pdT)84聚合物可以组装成一种新的固态聚合物，例如聚(dA)290。聚(/3H/dT)200，聚(dT)85-纤维素，反过来，它是用于检测DNA连接酶的非常方便的底物。
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