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尼龙管支撑的烟酰胺 - 腺嘌呤二核苷酸激酶的制备及性质

Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase.

作者信息

Sundaram P V, Apps D K

出版信息

Biochem J. 1977 Feb 1;161(2):441-3. doi: 10.1042/bj1610441.

Abstract

Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction changed upon immobilization, but the pH-dependence remained unaltered. The activities and stabilities of different preparations are compared.

摘要

鸽肝NAD激酶被共价偶联到尼龙管的内表面,既可以直接偶联到尼龙上,也可以通过多胺间隔物进行偶联。固定化后,反应的Km值和失活能发生了变化,但pH依赖性保持不变。对不同制剂的活性和稳定性进行了比较。

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