Novak R L, Dohnal J
Nucleic Acids Res. 1974 Jun;1(6):753-9. doi: 10.1093/nar/1.6.753.
Thermal denaturation studies show that 10-15% of the calf thymus DNA in the heat denatured (Tyr-Gly-Tyr-Gly-Tyr)-DNA complex renatures spontaneously after colling. The double-strandness of this DNA was verified by its resistance to single-strand Neurospora endonuclease and by its elution profile on hydroxypatite columns. The renatured DNA isolated by the latter technique was found to contain 56% GC compared to the 41% GC content of the whole thymus DNA. Alternating tryptophanyl-glycyl and histidyl-glycyl peptides also catalyze the same renaturation. A linear correlation was found between the thermal stabilization afforded to the DNA by the various peptides and their ability to "catalyze" DNA strand renaturation.
热变性研究表明,热变性的(酪氨酸-甘氨酸-酪氨酸-甘氨酸-酪氨酸)-DNA复合物中10%-15%的小牛胸腺DNA在冷却后会自发复性。这种DNA的双链性通过其对单链脉孢菌内切酶的抗性以及在羟基磷灰石柱上的洗脱图谱得到验证。通过后一种技术分离得到的复性DNA被发现含有56%的GC,而整个胸腺DNA的GC含量为41%。交替的色氨酸-甘氨酸和组氨酸-甘氨酸肽也能催化相同的复性过程。发现各种肽赋予DNA的热稳定性与其“催化”DNA链复性的能力之间存在线性相关性。