根据热稳定性、序列长度和复性速率对复性的重复人类DNA进行分级分离。

Fractionation of renatured repetitive human DNA according to thermal stability, sequence length, and renaturation rate.

作者信息

Houck C M, Rinehart F P, Schmid C W

出版信息

Biochim Biophys Acta. 1978 Mar 29;518(1):37-52. doi: 10.1016/0005-2787(78)90114-4.

Abstract

Renatured repetitive human DNA sequences have been fractionated according to three criteria: length, thermal stability, and rate of renaturation. Satellite sequences are found in the long repetitive sequence fractions and renatured satellite sequences are well base paired by the criterion of thermal stability. No satellites are observed in short repeated sequences. Short repeated sequences are interspersed with single copy sequences and are poorly base paired. This implies satellite components are relatively homogeneous in base sequence whereas short interspersed repeated sequences are inexact copies of each other. A method for isolating satellite DNAs based on their high thermal stabilities is discussed.

摘要

复性的重复人类DNA序列已根据三个标准进行了分级分离：长度、热稳定性和复性速率。卫星序列存在于长重复序列组分中，并且根据热稳定性标准，复性的卫星序列具有良好的碱基配对。在短重复序列中未观察到卫星序列。短重复序列与单拷贝序列相间分布，且碱基配对较差。这意味着卫星组分在碱基序列上相对均匀，而短散在重复序列彼此并非精确拷贝。本文讨论了一种基于其高热稳定性分离卫星DNA的方法。