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利用线粒体DNA串联重复序列对猫和狗进行个体识别?

Individual identification of cats and dogs using mitochondrial DNA tandem repeats?

作者信息

Fridez F, Rochat S, Coquoz R

机构信息

Institut de Police Scientifique et de Criminologie, University of Lausanne, Bâtiment de Chimie, CH-1015 Lausanne-Dorigny, Switzerland.

出版信息

Sci Justice. 1999 Jul-Sep;39(3):167-71. doi: 10.1016/S1355-0306(99)72042-3.

Abstract

Cats and dogs are very common animals in the human environment. In Switzerland, one in five households owns a cat or a dog. Their hairs are very easily transferred and could be used as a frequent trace evidence. Using DNA analysis, identification of these animals should be possible as it is in human identification. However, most of the time, no nuclear DNA can be recovered from the hair. It is therefore necessary to rely on mtDNA. Cats and dogs have tandemly repeated sequences in their mtDNA control region. In this study, the authors show that these tandem repeats are very polymorphic but are also the source of a very high level of heteroplasmy. The authors, therefore, examined if this might prevent their use in forensic identification.

摘要

猫和狗是人类环境中非常常见的动物。在瑞士,五分之一的家庭养猫或狗。它们的毛发很容易转移,可作为常见的微量证据。利用DNA分析,应该能够像对人类进行身份识别那样对这些动物进行识别。然而,大多数时候,无法从毛发中提取到核DNA。因此有必要依靠线粒体DNA。猫和狗的线粒体DNA控制区存在串联重复序列。在本研究中,作者表明这些串联重复序列具有高度多态性,但也是高度异质性的来源。因此,作者研究了这是否会妨碍它们在法医鉴定中的应用。

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