Tarditi Christy R, Grahn Robert A, Evans Jeffrey J, Kurushima Jennifer D, Lyons Leslie A
Department of Population Health and Reproduction, University of California, Davis, 95616, USA.
J Forensic Sci. 2011 Jan;56 Suppl 1(Suppl 1):S36-46. doi: 10.1111/j.1556-4029.2010.01592.x. Epub 2010 Nov 15.
Approximately 81.7 million cats are in 37.5 million U.S. households. Shed fur can be criminal evidence because of transfer to victims, suspects, and/or their belongings. To improve cat hairs as forensic evidence, the mtDNA control region from single hairs, with and without root tags, was sequenced. A dataset of a 402-bp control region segment from 174 random-bred cats representing four U.S. geographic areas was generated to determine the informativeness of the mtDNA region. Thirty-two mtDNA mitotypes were observed ranging in frequencies from 0.6-27%. Four common types occurred in all populations. Low heteroplasmy, 1.7%, was determined. Unique mitotypes were found in 18 individuals, 10.3% of the population studied. The calculated discrimination power implied that 8.3 of 10 randomly selected individuals can be excluded by this region. The genetic characteristics of the region and the generated dataset support the use of this cat mtDNA region in forensic applications.
美国约3750万户家庭中养有大约8170万只猫。脱落的毛发可能成为犯罪证据，因为毛发会转移到受害者、嫌疑人及/或他们的物品上。为了提升猫毛作为法医证据的价值，对带有和不带有毛囊的单根毛发的线粒体DNA控制区进行了测序。生成了一个数据集，该数据集来自代表美国四个地理区域的174只随机繁殖猫的402个碱基对控制区片段，以确定线粒体DNA区域的信息含量。观察到32种线粒体DNA单倍型，频率范围为0.6%至27%。所有群体中都出现了四种常见类型。确定低异质性为1.7%。在18只个体（占所研究群体的10.3%）中发现了独特的单倍型。计算得出的鉴别力表明，该区域可以排除10个随机选择个体中的8.3个。该区域的遗传特征以及生成的数据集支持在法医应用中使用这个猫线粒体DNA区域。
