De Marco P, Murrell J C, Bordalo A A, Moradas-Ferreira P
IBMC, Universidade do Porto, Portugal.
Arch Microbiol. 2000 Feb;173(2):146-53. doi: 10.1007/s002039900124.
Two novel bacterial strains that can utilize methanesulfonic acid as a source of carbon and energy were isolated from a soil sample collected in northern Portugal. Morphological, physiological, biochemical and molecular biological characterization of the two isolates indicate that strain P1 is a pink-pigmented facultative methylotroph belonging to the genus Methylobacterium, while strain P2 is a restricted methylotroph belonging to the genus Hyphomicrobium. Both strains are strictly aerobic, degrade methanesulfonate, and release small quantities of sulfite into the medium. Growth on methanesulfonate induces a specific polypeptide profile in each strain. This, together with the positive hybridization to a DNA probe that carries the msm genes of Methylosulfonomonas methylovora strain M2, strongly endorses the contention that a methanesulfonic acid monooxygenase related to that found in the previously known methanesulfonate-utilizing bacteria is present in strains P1 and P2. The isolation of bacteria containing conserved msm genes from diverse environments and geographical locations supports the hypothesis that a common enzyme may be globally responsible for the oxidation of methanesulfonate by natural methylotrophic communities.
从葡萄牙北部采集的土壤样本中分离出了两种能够利用甲磺酸作为碳源和能源的新型细菌菌株。对这两种分离菌株进行的形态学、生理学、生物化学和分子生物学特征分析表明，菌株P1是一种属于甲基杆菌属的粉红色兼性甲基营养菌，而菌株P2是一种属于生丝微菌属的受限甲基营养菌。两种菌株均为严格需氧菌，可降解甲磺酸盐，并向培养基中释放少量亚硫酸盐。在甲磺酸盐上生长会在每种菌株中诱导出特定的多肽谱。这一点，再加上与携带甲基磺酸甲基单胞菌菌株M2的msm基因的DNA探针的阳性杂交，有力地支持了这样一种观点，即菌株P1和P2中存在一种与先前已知的利用甲磺酸盐的细菌中发现的甲磺酸单加氧酶相关的酶。从不同环境和地理位置分离出含有保守msm基因的细菌，支持了这样一种假说，即一种共同的酶可能在全球范围内负责天然甲基营养群落对甲磺酸盐的氧化。
