Satake M, Ichimura T, Sekiguchi K, Yoshimatsu S, Oshima Y
Department of Applied Bioorganic Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Nat Toxins. 1999;7(4):147-50. doi: 10.1002/(sici)1522-7189(199907/08)7:4<147::aid-nt50>3.0.co;2-6.
Two different strains of the dinoflagellate Protoceratium reticulatum collected at Harima Nada and Yamada Bay in Japan were cultured and analyzed by fluorometric HPLC for yessotoxin production. Only the Yamada Bay strain produced yessotoxin. The toxin together with its analog, 45,46,47-trinoryessotoxin, were isolated from larger scale culture and unambiguously confirmed by (1)H NMR and MS measurements. This is the first confirmation of the biogenetic origin of yessotoxin in Japan, where the toxin was first reported. The results also indicate that the production of yessotoxins by P. reticulatum differs from strain to strain, in a similar way to that observed in many other toxigenic dinoflagellates such as Dinophysis spp. and Alexandrium spp.
对从日本播磨滩和山田湾采集的两种不同品系的网状原甲藻进行培养，并通过荧光高效液相色谱法分析其产岩藻毒素的情况。只有山田湾品系产生岩藻毒素。从大规模培养物中分离出该毒素及其类似物45,46,47-三去甲岩藻毒素，并通过¹H NMR和质谱测量进行了明确确认。这是在日本首次证实岩藻毒素的生物起源，日本是该毒素首次被报道的地方。结果还表明，网状原甲藻产岩藻毒素的情况因品系而异，这与在许多其他产毒甲藻如鳍藻属和亚历山大藻属中观察到的情况类似。
