Smith P G
J Membr Biol. 1975;22(2):165-81. doi: 10.1007/BF01868169.
The resistance and capacitance of the isolated amphibian skin have been determined from measurements of the response of the voltage across the skin to small steps to current. Previous work indicates that the electrical impedance of frog skin, when the skin is bathed with Ringer's solution on both sides, is largely determined by the properties of the functional outward-facing membrane of the skin, the outer membrane of the stratum granulosum (P.G. Smith, 1971, Acta Physiol. Scand. 81:355). This membrane can be represented by a resistance and capacitance in parallel. Aldosterone, which induces conversion of the s. granulosum into a cornified cell layer and transformation of the cell layer below into a new s. granulosum, also causes a transient rise in resistance and a short-lived decrease in capacitance to about one-half its initial value. It is suggested that these electrical changes are caused by the transitory presence of two functional outward-facing membranes in series. The method of determining resistance and capacitance from the voltage response is discussed in the Appendix.
通过测量施加于皮肤的小电流阶跃所引起的皮肤跨膜电压响应，已测定出离体两栖类皮肤的电阻和电容。先前的研究表明，当青蛙皮肤两侧均用林格氏液浸润时，其电阻抗在很大程度上由皮肤功能外向膜（颗粒层外层膜）的特性所决定（P.G. 史密斯，1971年，《生理学杂志》81卷：355页）。该膜可用一个并联的电阻和电容来表示。醛固酮可诱导颗粒层转变为角质化细胞层，并使下方的细胞层转变为新的颗粒层，同时还会导致电阻瞬间升高以及电容短暂下降至初始值的约二分之一。有人认为，这些电学变化是由两个串联的功能性外向膜的短暂存在所引起的。从电压响应确定电阻和电容的方法在附录中进行了讨论。
