Büchs J, Maier U, Milbradt C, Zoels B
Chair of Biochemical Engineering, Sammelbau, Aachen University of Technology, D-52074 Aachen, Germany.
Biotechnol Bioeng. 2000 Jun 20;68(6):589-93. doi: 10.1002/(sici)1097-0290(20000620)68:6<589::aid-bit1>3.0.co;2-j.
In this first article of a series a new method is introduced that enables the accurate determination of the power consumption in a shaking flask. The method is based on torque measurements in the drive and appropriate compensation of the friction losses. The results for unbaffled shaking flasks at low viscosities are presented after varying shaking frequency, flask size, filling volume, shaking diameter, and surface quality (hydrophilic and hydrophobic) of the inner flask walls. The order of magnitude of the values of power consumption in shaking flasks is equal to, or even higher than, the values typical for agitated tank bioreactors. A physically based model equation for shaking flasks is derived that introduces a modified power number and a resulting constant as the only fitting parameter. With this equation, the measured results are correlated with sufficient accuracy. For the first time, comprehensive data for the power consumption in unbaffled shaking flasks at low viscosity is available, giving a detailed picture of the influences of the different variables.
在本系列的第一篇文章中，介绍了一种新方法，该方法能够准确测定摇瓶中的功耗。该方法基于对驱动器中的扭矩测量以及对摩擦损失的适当补偿。在改变摇瓶频率、摇瓶尺寸、填充体积、摇动直径和内瓶壁的表面质量（亲水和疏水）后，给出了低粘度下无挡板摇瓶的结果。摇瓶中功耗值的数量级等于甚至高于搅拌罐式生物反应器的典型值。推导了一个基于物理的摇瓶模型方程，该方程引入了修正功率数和一个结果常数作为唯一的拟合参数。利用该方程，测量结果具有足够的相关性。首次获得了低粘度下无挡板摇瓶功耗的综合数据，详细描述了不同变量的影响。
