Maier U, Büchs J
Department of Biochemical Engineering, Aachen University of Technology (RWTH), Sammelbau Biologie, D-52074, Aachen, Germany
Biochem Eng J. 2001 Mar;7(2):99-106. doi: 10.1016/s1369-703x(00)00107-8.
The maximum gas-liquid mass transfer capacity of 250ml shaking flasks on orbital shaking machines has been experimentally investigated using the sulphite oxidation method under variation of the shaking frequency, shaking diameter, filling volume and viscosity of the medium. The distribution of the liquid within the flask has been modelled by the intersection between the rotational hyperboloid of the liquid and the inner wall of the shaking flask. This model allows for the calculation of the specific exchange area (a), the mass transfer coefficient (k(L)) and the maximum oxygen transfer capacity (OTR(max)) for given operating conditions and requires no fitting parameters. The model agrees well with the experimental results. It was furthermore shown that the liquid film on the flask wall contributes significantly to the specific mass transfer area (a) and to the oxygen transfer rate (OTR).
使用亚硫酸盐氧化法，在摇床频率、摇床直径、填充体积和介质粘度变化的情况下，对250毫升摇瓶在轨道摇床上的最大气液传质能力进行了实验研究。摇瓶内液体的分布通过液体旋转双曲面与摇瓶内壁的交点进行建模。该模型允许计算给定操作条件下的比交换面积（a）、传质系数（k（L））和最大氧传递能力（OTR（max）），且无需拟合参数。该模型与实验结果吻合良好。此外还表明，摇瓶壁上的液膜对比传质面积（a）和氧传递速率（OTR）有显著贡献。
