[DNA疫苗或灭活疫苗诱导的抗蜱传脑炎免疫反应的比较]

[A comparison of the immune response induced by DNA or by an inactivated vaccine against tick-borne encephalitis].

作者信息

Morozova O V, Popova R V, Maksimova T G, Mitrofanova E E, Bakhvalova V N

机构信息

Institute of Bioorganic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2000 Mar-Apr(2):54-7.

PMID:10808575

Abstract

BALB/c mice were immunized with recombinant plasmid DNA pSVK3-ENS1 and pcDNAI-NS3 containing, respectively, genes E-NS1 and NS3 of tick-borne encephalitis (TBE) virus. Antibodies to TBE virus proteins were detected in the blood sera of the immunized animals by the method of the enzyme immunoassay. Though the titers of virus-specific antibodies in the sera of mice immunized with protein vaccines exceeded those registered after immunization with DNA vaccines, essential protective immunity was observed after the use of both vaccines.

摘要

用分别含有蜱传脑炎（TBE）病毒E-NS1和NS3基因的重组质粒DNA pSVK3-ENS1和pcDNAI-NS3对BALB/c小鼠进行免疫。通过酶免疫测定法在免疫动物的血清中检测到针对TBE病毒蛋白的抗体。虽然用蛋白疫苗免疫的小鼠血清中病毒特异性抗体的滴度超过了用DNA疫苗免疫后记录的滴度，但使用这两种疫苗后均观察到了基本的保护性免疫。