Timofeev A V, Ozherelkov S V, Pronin A V, Deeva A V, Karganova G G, Elbert L B, Stephenson J R
Chumakov Institute of Poliomyelitis and Viral Encephalitides RAMS, Moscow Region, Russia.
J Gen Virol. 1998 Apr;79 ( Pt 4):689-95. doi: 10.1099/0022-1317-79-4-689.
The humoral immune response to flaviviruses is mainly directed to the major envelope protein, E, and a glycosylated non-structural protein, NS1. Cell-mediated immune responses, however, appear to be directed mainly against non-structural proteins. Experiments described here show that a defective recombinant adenovirus (Rad51) containing the gene encoding the NS1 protein of tick-borne encephalitis virus can induce a strong protective immune response against several pathogenic tick-borne flaviviruses in an experimental animal model, and can enhance the efficacy of conventional vaccine preparations. A protective immune response against a lethal virus challenge can also be induced by the passive transfer of antibodies, B cells or T cells from animals vaccinated with Rad51. Raised levels of non-neutralizing antibodies and cytokines associated with a T helper cell-type 1 immune response are also observed. These data demonstrate the importance of non-structural viral proteins in the protective immune response against flaviviruses and support the use of non-structural viral proteins as vaccine components.
针对黄病毒的体液免疫反应主要针对主要包膜蛋白E和一种糖基化非结构蛋白NS1。然而，细胞介导的免疫反应似乎主要针对非结构蛋白。此处所述实验表明，一种含有蜱传脑炎病毒NS1蛋白编码基因的缺陷重组腺病毒（Rad51），在实验动物模型中可诱导针对几种致病性蜱传黄病毒的强烈保护性免疫反应，并可提高传统疫苗制剂的效力。用Rad51疫苗接种的动物的抗体、B细胞或T细胞的被动转移也可诱导针对致命病毒攻击的保护性免疫反应。还观察到与1型辅助性T细胞免疫反应相关的非中和抗体和细胞因子水平升高。这些数据证明了病毒非结构蛋白在针对黄病毒的保护性免疫反应中的重要性，并支持将病毒非结构蛋白用作疫苗成分。
