铬（III）配合物对DNA的切割作用。
DNA cleavage by a Chromium(III) complex.
作者信息
Vijayalakshmi R, Kanthimathi M, Subramanian V, Nair B U
机构信息
Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai, 600 020, India.
出版信息
Biochem Biophys Res Commun. 2000 May 19;271(3):731-4. doi: 10.1006/bbrc.2000.2707.
A new Cr(III) complex with the empirical formula [Cr(Schiff base) (H(2)O)(2)]ClO(4), where the Schiff base is 2, 3-bis¿[(2-hydroxy-4-diethylamino) (phenyl) (methylene)]amino¿2-butenedinitrile has been synthesized and characterized spectroscopically. Binding of this complex to DNA has been studied using UV-visible spectroscopy. The complex has been found to bind to the major groove of DNA with a binding constant, K = (1.3 +/- 0.2) x 10(3) M(-1). The induced CD spectrum of the complex in the presence of DNA is also indicative of major groove binding. Gel electrophoresis of plasmid DNA in the presence of the complex shows that the complex brings about nicking of the DNA.
一种经验式为[Cr(席夫碱)(H₂O)₂]ClO₄的新型三价铬配合物已被合成并通过光谱进行了表征，其中席夫碱为2,3 - 双 - [(2 - 羟基 - 4 - 二乙氨基)(苯基)(亚甲基)]氨基 - 2 - 丁烯二腈。已使用紫外可见光谱研究了该配合物与DNA的结合。发现该配合物以结合常数K = (1.3 ± 0.2)×10³ M⁻¹与DNA的大沟结合。在DNA存在下该配合物的诱导圆二色光谱也表明其与大沟结合。在该配合物存在下质粒DNA的凝胶电泳显示该配合物会导致DNA切口。
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