Fujita S, Litwin S D, Hartman N
J Exp Med. 1975 Dec 1;142(6):1416-24. doi: 10.1084/jem.142.6.1416.
A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.
本文描述了一种利用差速离心和蔗糖密度梯度离心从人淋巴细胞中分离膜组分的方法。对来自长期培养的人B淋巴细胞系、T淋巴细胞系、外周血淋巴细胞、扁桃体淋巴细胞和胸腺细胞的膜制备物在0.5%十二烷基硫酸钠-7.5%聚丙烯酰胺凝胶上进行分析，该凝胶用于蛋白质和碳水化合物染色。最重要的发现是，与B淋巴细胞的膜制备物相关的一种约30,000道尔顿的主要糖蛋白。T淋巴细胞制备物中未含有易于检测到的这种膜相关成分。T细胞系MOLT-4很独特，因为它在约30,000道尔顿处有一条窄的蛋白带，该蛋白带不含碳水化合物。
