Gahmberg C G, Häyry P, Andersson L C
J Cell Biol. 1976 Mar;68(3):642-53. doi: 10.1083/jcb.68.3.642.
We have labeled exposed surface glycoproteins of mouse lymphoid cells by the galactose oxidase-tritated sodium borohydride technique. The labeled glyco-proteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography (fluorography). The major thymocyte surface proteins have molecular weights of 170,000 and 125,000. Thymocytes from TL antigen-positive mouse strains showed an additional band with a molecular weight of 27,000. Highly purified T lymphocytes contain two major surface glycoproteins with molecular weights of 180,000 and 125,000. Purified B lymphocytes have one major surface glycoprotein with a molecular weight of 210,000. When T lymphocytes are stimulated in vitro by concanavalin A or phytohemag-glutinin, the major proteins characteristic of T cells are relatively weakly labeled, but new components of lower molecular weights appear on the cell surface. A similar change is seen in B lymphocytes stimulated by Escherichia coli lipopolysaccharide. T lymphoblasts isolated from mixed lymphocyte cultures show a slightly different surface glycoprotein pattern. A polypeptide with a molecular weight of 57,000, which was labeled without enzymatic treatment by tritiated sodium borohydride alone, is strongly labeled in proliferating cells.
我们运用半乳糖氧化酶-氚化硼氢化钠技术标记了小鼠淋巴细胞的暴露表面糖蛋白。通过聚丙烯酰胺平板凝胶电泳分离标记的糖蛋白，并通过放射自显影（荧光自显影）进行可视化。主要的胸腺细胞表面蛋白分子量为170,000和125,000。来自TL抗原阳性小鼠品系的胸腺细胞显示出一条分子量为27,000的额外条带。高度纯化的T淋巴细胞含有两种主要的表面糖蛋白，分子量分别为180,000和125,000。纯化的B淋巴细胞有一种主要的表面糖蛋白，分子量为210,000。当T淋巴细胞在体外受到刀豆球蛋白A或植物血凝素刺激时，T细胞特有的主要蛋白标记较弱，但细胞表面会出现分子量较低的新成分。在受到大肠杆菌脂多糖刺激的B淋巴细胞中也观察到类似变化。从混合淋巴细胞培养物中分离出的T淋巴母细胞显示出略有不同的表面糖蛋白模式。一种分子量为57,000的多肽，仅通过氚化硼氢化钠在无酶处理的情况下被标记，在增殖细胞中被强烈标记。
