Hrabák A, Antoni F, Szabó M T
Acta Biochim Biophys Acad Sci Hung. 1977;12(3):245-58.
A microsomal fraction containing high amounts of plasma membrane has been isolated from human tonsillar lymphocytes. The subcellular fractions were characterized on the basis of their chemical composition and enzyme activities. A glycoprotein fraction solubilized by lithium-diiodo-salicylate (LIS) was purified by ion-exchange chromatography. The purified glycoprotein was analyzed by various methods and its carbohydrate content was determined. The glycoprotein contained large amounts of hexoses and sialic acid and this fraction represented 3--4% of the whole plasma membrane protein. The fraction was shown to consist of three individual proteins (4 X 10(4), 4.6 X 10(4) and 4.8 X 10(4) daltons) determined by SDS-polyacrylamide gel electrophoresis.
已从人扁桃体淋巴细胞中分离出含有大量质膜的微粒体部分。根据其化学组成和酶活性对亚细胞部分进行了表征。用锂二碘水杨酸（LIS）溶解的糖蛋白部分通过离子交换色谱法进行纯化。通过各种方法对纯化的糖蛋白进行分析并测定其碳水化合物含量。该糖蛋白含有大量己糖和唾液酸，且该部分占整个质膜蛋白的3 - 4%。通过SDS - 聚丙烯酰胺凝胶电泳确定该部分由三种个体蛋白（4×10⁴、4.6×10⁴和4.8×10⁴道尔顿）组成。
